Journal: Experimental and Therapeutic Medicine

Article Title: Involvement of neurotrophic signaling in doxorubicin-induced cardiotoxicity

doi: 10.3892/etm.2019.8276

Figure Lengend Snippet: Effects of DOX on the expression of BDNF and NGF in the serum and heart. (A) BDNF concentration in the serum. (B) BDNF mRNA levels in the heart. (C) BDNF protein expression levels in the heart. (D) NGF concentration in the serum. (E) NGF mRNA levels in the heart. (F) NGF protein levels in the heart. Data are expressed as the mean ± standard deviation (n=8–9). *P<0.05 and **P<0.01 compared with Con. NGF, nerve growth factor; BDNF, brain derived neurotrophic factor; DOX, doxorubicin; Con, control.

Article Snippet: Determination of serum BDNF and NGF levels ELISA kits were purchased from Wuhan USCN Business Co., Ltd. and were used to determine plasma levels of BDNF (cat. no. SEA011Ra) and NGF (cat.no.

Techniques: Expressing, Concentration Assay, Standard Deviation, Derivative Assay

Journal: Brain Sciences

Article Title: The Impact of Intermittent Fasting on Brain-Derived Neurotrophic Factor, Neurotrophin 3, and Rat Behavior in a Rat Model of Type 2 Diabetes Mellitus

doi: 10.3390/brainsci11020242

Figure Lengend Snippet: Neurotrophin 3 (NT3) ( A ) and brain-derived neurotrophic factor (BDNF) ( B ) level in the hippocampus in all groups of rats. Data were expressed as mean ± standard deviation (SD) of n = 10 rats/group. Values were considered significantly different at p < 0.05. *: significantly different as compared to control (ad libitum). #: significantly different as compared to intermittent fasting (IF) group. $: significantly different as compared to type 2 diabetes mellitus (T2DM) group.

Article Snippet: ELISA assay kits were used for the determination of BDNF (catalog number: MBS2700741, MyBioSource, San Diego, CA, USA), NT3 (catalog number: MBS2701228, MyBioSource, USA), 5-HT (catalog number: MBS725497, MyBioSource, USA), dopamine (catalog number: MBS701755, MyBioSource, USA), and glutamic acid (catalog number: MBS2700682, MyBioSource, San Diego, CA, USA) levels in the hippocampus according to the manufacturer’s instructions.

Techniques: Derivative Assay, Standard Deviation, Control

Journal: Brain Sciences

Article Title: The Impact of Intermittent Fasting on Brain-Derived Neurotrophic Factor, Neurotrophin 3, and Rat Behavior in a Rat Model of Type 2 Diabetes Mellitus

doi: 10.3390/brainsci11020242

Figure Lengend Snippet: ( A ) Pearson correlation between HOMA-IR and NT3 level in the hippocampus, ( B ) Pearson correlation between HOMA-IR and BDNF level in the hippocampus, and ( C ) Pearson correlation between serum corticosterone and 5-HT level in the hippocampus. Neurotrophin3: NT3 *: p < 0.05, **: p < 0.01, ****: p < 0.0001.

Article Snippet: ELISA assay kits were used for the determination of BDNF (catalog number: MBS2700741, MyBioSource, San Diego, CA, USA), NT3 (catalog number: MBS2701228, MyBioSource, USA), 5-HT (catalog number: MBS725497, MyBioSource, USA), dopamine (catalog number: MBS701755, MyBioSource, USA), and glutamic acid (catalog number: MBS2700682, MyBioSource, San Diego, CA, USA) levels in the hippocampus according to the manufacturer’s instructions.

Techniques:

Journal: Biology

Article Title: Exploring the Effect of Acute and Regular Physical Exercise on Circulating Brain-Derived Neurotrophic Factor Levels in Individuals with Obesity: A Comprehensive Systematic Review and Meta-Analysis

doi: 10.3390/biology13050323

Figure Lengend Snippet: Full search strategy for each database.

Article Snippet: Zibinaite et al., 2019 [ ] , RCT , 26 , Sedentary women , EG: 13 CG: 13 , AE: 72 supervised exercise sessions on cycle ergometers over 6 months, 3 sessions per week. Each session lasted 50 min at an intensity between 60% and 70% of HRmax. CG: maintained their own life-styles with no intervention , Serum BDNF (pg/mL) , Human BDNF Elisa Kits (Gemini; Stratec Biomedical, Birkenfeld, Germany). , EG: Unchanged CG: Unchanged.

Techniques: Derivative Assay

Journal: Biology

Article Title: Exploring the Effect of Acute and Regular Physical Exercise on Circulating Brain-Derived Neurotrophic Factor Levels in Individuals with Obesity: A Comprehensive Systematic Review and Meta-Analysis

doi: 10.3390/biology13050323

Figure Lengend Snippet: Characteristics of the included studies for regular exercise on circulating BDNF level (N: 13, 17 trials) (Continuation of Table 3 ).

Article Snippet: Zibinaite et al., 2019 [ ] , RCT , 26 , Sedentary women , EG: 13 CG: 13 , AE: 72 supervised exercise sessions on cycle ergometers over 6 months, 3 sessions per week. Each session lasted 50 min at an intensity between 60% and 70% of HRmax. CG: maintained their own life-styles with no intervention , Serum BDNF (pg/mL) , Human BDNF Elisa Kits (Gemini; Stratec Biomedical, Birkenfeld, Germany). , EG: Unchanged CG: Unchanged.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Biology

Article Title: Exploring the Effect of Acute and Regular Physical Exercise on Circulating Brain-Derived Neurotrophic Factor Levels in Individuals with Obesity: A Comprehensive Systematic Review and Meta-Analysis

doi: 10.3390/biology13050323

Figure Lengend Snippet: Forest plot illustrating the effect of acute exercise on circulating BDNF levels in comparison to controls. Forest plot values are shown as effect sizes (Hedges’ g) with 95% confidence intervals (CI). Black squares: individual studies. The size represents the relative weight. Black rhomboid: summary value. Mean results: ES = 1.25, 95% CI = 0.19 to 2.30, p = 0.021, I 2 = 80.4%, N total participants = 104 [ , , ].

Article Snippet: Zibinaite et al., 2019 [ ] , RCT , 26 , Sedentary women , EG: 13 CG: 13 , AE: 72 supervised exercise sessions on cycle ergometers over 6 months, 3 sessions per week. Each session lasted 50 min at an intensity between 60% and 70% of HRmax. CG: maintained their own life-styles with no intervention , Serum BDNF (pg/mL) , Human BDNF Elisa Kits (Gemini; Stratec Biomedical, Birkenfeld, Germany). , EG: Unchanged CG: Unchanged.

Techniques: Comparison

Journal: Biology

Article Title: Exploring the Effect of Acute and Regular Physical Exercise on Circulating Brain-Derived Neurotrophic Factor Levels in Individuals with Obesity: A Comprehensive Systematic Review and Meta-Analysis

doi: 10.3390/biology13050323

Figure Lengend Snippet: Forest plot illustrating the effect of regular exercise intervention on circulating BDNF levels in comparison to controls. Forest plot values are shown as effect sizes (Hedges’ g) with 95% confidence intervals (CI). Black squares: individual studies. The size represents the relative weight. Black rhomboid: summary value. Mean results: ES = 0.49, 95% CI = −0.08 to 1.06, p = 0.089, I 2 = 88.7%, N total participants = 571 [ , , , , , , , , , , , , ].

Article Snippet: Zibinaite et al., 2019 [ ] , RCT , 26 , Sedentary women , EG: 13 CG: 13 , AE: 72 supervised exercise sessions on cycle ergometers over 6 months, 3 sessions per week. Each session lasted 50 min at an intensity between 60% and 70% of HRmax. CG: maintained their own life-styles with no intervention , Serum BDNF (pg/mL) , Human BDNF Elisa Kits (Gemini; Stratec Biomedical, Birkenfeld, Germany). , EG: Unchanged CG: Unchanged.

Techniques: Comparison

Journal: NPJ Vaccines

Article Title: A multi-targeting immunotherapy ameliorates multiple facets of Alzheimer’s disease in 3xTg mice

doi: 10.1038/s41541-024-00942-9

Figure Lengend Snippet: a Methods for pathological evaluation: Outline of methods for evaluating mouse brain pathology after vaccine immunization. b Aβ Immunostaining: 6E10 immunostaining for Aβ burden in 3xTg mice brains. (Black scale bar: 100 μm). c Quantification of Aβ immunostaining: Quantification of 6E10 immunostaining in the hippocampal CA1 and cortex regions. d Aβ42 analysis: Aβ42 levels in soluble and insoluble brain homogenates by flow cytometry (The experimental samples are mixed samples ( n = 9–10) and repeated three times). e pTau Immunostaining: AT8 immunostaining for phosphorylated-tau in 3xTg mice brains. (Red scale bar: 50 μm). f Quantification of pTau immunostaining: Quantification of AT8 immunostaining in the hippocampal CA1 and cortex regions ( n = 9–10). g pTau202/205 analysis: pTau202/205 levels in soluble and insoluble brain homogenates by western blot (Mixed samples, n = 9–10). GAPDH served as the internal control. The relative content of each sample is marked with a red number. h PHF-13 Immunostaining: PHF-13 immunostaining for phosphorylated-tau in 3xTg mice brains. (Red scale bar: 50 μm). i Quantification of PHF-13 immunostaining: Quantification of PHF-13 immunostaining in the hippocampal DG and cortex regions ( n = 9–10). j pTau396 analysis: pTau396 levels in soluble and insoluble brain homogenates by western blot (Mixed samples, n = 9–10). GAPDH served as the internal control. The relative content of each sample is marked with a red number. k Phospho-tau (Ser404) immunostaining: Immunostaining for phosphorylated-tau (Ser404) in 3xTg mice brains. (Red scale bar: 50 μm). l Quantification of phospho-tau (Ser404) immunostaining: Quantification of phospho-tau (Ser404) immunostaining in hippocampal CA1 and cortex regions ( n = 9–10). m pTau404 analysis: pTau404 levels in soluble and insoluble brain homogenates by western blot (Mixed samples, n = 9–10). GAPDH served as the internal control. The relative content of each sample is marked with a red number. *Data presented as mean ± SEM. Multivariate ANOVA was employed when p ≤ 0.05. Bonferroni-corrected t -test was otherwise applied. Statistical significance denoted as * p < 0.05, ** p < 0.01, *** p < 0.001; ns not significant. (Fig. 2a was created with BioRender.com).

Article Snippet: The levels of Aβ42 in serum and brain homogenates (both soluble and insoluble fractions) of 3xTg mice were determined using Human Aβ42 ELISA kits (Uscn Life Science Inc., China), following the manufacturer’s protocol.”

Techniques: Immunostaining, Flow Cytometry, Western Blot, Control

Journal: NPJ Vaccines

Article Title: A multi-targeting immunotherapy ameliorates multiple facets of Alzheimer’s disease in 3xTg mice

doi: 10.1038/s41541-024-00942-9

Figure Lengend Snippet: a 6E10 immunostaining for Aβ burden: Immunostaining for Aβ burden in the brains of 3xTg mice treated with vaccines or vehicle. (White scale bar: 250 μm). b Quantification of Aβ immunostaining: quantification of 6E10 immunostaining in the hippocampus and cortex regions ( n = 13–15). c , d Aβ42 analysis: Aβ42 levels in soluble and insoluble brain homogenates in the hippocampus. ( c ) and cortex regions ( d ) by flow cytometry (The experimental samples are mixed samples ( n = 13–15) and repeated three times). e PHF-13 Immunostaining for pTau: Immunostaining for phosphorylated-tau (PHF-13) in the brains of 3xTg mice treated with vaccines or vehicle (Red scale bar: 50 μm). f Quantification of PHF-13 immunostaining: Quantification of PHF-13 immunostaining in the hippocampal DG and Cortex regions ( n = 13–15). g , h pTau396 analysis: pTau396 levels in soluble and insoluble brain homogenates in the hippocampal DG ( g ) and Cortex regions ( h ) by western blot ( n = 13–15). GAPDH served as the internal control. The relative content of each sample is marked with a red number. i Hindlimb clamping (Clasp Score): Clasp scores in hindlimb clamping ( n = 13–15 per group, one-way ANOVA). j Novel object recognition (NOR): Cognitive functions assessed in novel object recognition ( n = 13–15 per group, one-way ANOVA). k Morris water maze—escape latency: Escape latency to the platform during the training trials in the Morris water maze ( n = 13–15 per group, two-way ANOVA). l Morris water maze—platform crossings: Numbers of platform location crossings in the probe trial of Morris water maze ( n = 13–15 per group, one-way ANOVA). *Data were mean ± SEM. Multivariate ANOVA was employed when p ≤ 0.05. Bonferroni-corrected t -test was otherwise applied. * p < 0.05, ** p < 0.01, *** p < 0.001; ns not significant.

Article Snippet: The levels of Aβ42 in serum and brain homogenates (both soluble and insoluble fractions) of 3xTg mice were determined using Human Aβ42 ELISA kits (Uscn Life Science Inc., China), following the manufacturer’s protocol.”

Techniques: Immunostaining, Vaccines, Flow Cytometry, Western Blot, Control

Journal: NPJ Vaccines

Article Title: A multi-targeting immunotherapy ameliorates multiple facets of Alzheimer’s disease in 3xTg mice

doi: 10.1038/s41541-024-00942-9

Figure Lengend Snippet: a GFAP immunostaining in the hippocampus of premorbid 3xTg mice treated with the dual vaccine or PP vehicle (Vector). (White scale bar: 250 μm, Red scale bar: 50 μm). b Quantification of GFAP immunostaining in the hippocampus. c Representative Iba-1 images and microglia quantification: Representative Iba-1 images and quantification of branching and endpoint of microglia in the hippocampus. n = 7 mice per genotype with 6–8 microglia quantified per mouse. (Black scale bar: 100 μm, Red scale bar: 50 μm) d , e Microglial morphology analysis: Quantitative assessment of branching ( d ) and endpoint ( e ) of microglia. f Cytokine levels in brain lysates: Levels of TNF-α (left) and IL-1β (right) in the brain lysates of 3xTg mice determined using corresponding ELISA kits. ( n = 6–7). The experimental samples were mixed ( n = 6–7) and repeated three times. g , h Western blot analysis: Western blot analysis of GFAP, Iba-1, and GAPDH in the brain lysates of 3xTg mice treated with the dual vaccine or vehicle. GAPDH served as the internal control. The relative content of each sample is marked with a red number. *Data presented as mean ± SEM. Multivariate ANOVA was employed when p ≤ 0.05. Bonferroni-corrected t -test was otherwise applied. Statistical significance denoted as * p < 0.05, ** p < 0.01, *** p < 0.001; ns not significant.

Article Snippet: The levels of Aβ42 in serum and brain homogenates (both soluble and insoluble fractions) of 3xTg mice were determined using Human Aβ42 ELISA kits (Uscn Life Science Inc., China), following the manufacturer’s protocol.”

Techniques: Immunostaining, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Western Blot, Control

Journal: NPJ Vaccines

Article Title: A multi-targeting immunotherapy ameliorates multiple facets of Alzheimer’s disease in 3xTg mice

doi: 10.1038/s41541-024-00942-9

Figure Lengend Snippet: a , b Antibody concentrations in the brain: Concentrations of antibodies against Aβ and individual phosphorylated Tau sites in the brain of 3xTg mice from the premorbid group ( a ) and onset group ( b ). c , d Vaccine-generated antibodies impact on Aβ42: Vaccine-generated antibodies entering the brain and altering the Aβ42 content in the brain and peripheral blood of mice after immunization in premorbid group ( c ) and onset group ( d ). *Data presented as mean ± SEM. Multivariate ANOVA was employed when p ≤ 0.05. Bonferroni-corrected t -test was otherwise applied. Statistical significance denoted as * p < 0.05, ** p < 0.01, *** p < 0.001; ns not significant.

Article Snippet: The levels of Aβ42 in serum and brain homogenates (both soluble and insoluble fractions) of 3xTg mice were determined using Human Aβ42 ELISA kits (Uscn Life Science Inc., China), following the manufacturer’s protocol.”

Techniques: Generated

Journal: Frontiers in Pharmacology

Article Title: Tibetan mineral-herbal medicine Zuotai alleviates the depressive-like behaviors in chronic restraint-stressed mice while regulating stress hormone, inflammation and monoamine

doi: 10.3389/fphar.2023.1098378

Figure Lengend Snippet:

Article Snippet: Soluble starch (AR, Sinopharm Chemical, Shanghai, China), imipramine hydrochloride (113-52-0, 98%; 3B Scientific, Hamburg, Germany), sucrose (AR, Tianjin Beichen Fangzheng Reagent Factory, Tianjian, China), paraformaldehyde (AR, Tianjin Guangfu Institute of Fine Chemical Industry, Tianjin, China), mouse IL-6 ELISA kits (85-BMS603/2; eBioscience, CA, USA), mouse IL-6 (ELM-IL6-1, Raybiotech, GA, USA), mouse IL-1β ELISA kits (85-BMS6002; eBioscience, CA, USA), mouse IL-1β (ELM-IL1b-1, Raybiotech, GA, USA), mouse TNF-α ELISA kits (85-BMS607/3, eBioscience, CA, USA), and mouse TNF-α (ELM-TNFa-1, Raybiotech, GA, USA), norepinephrine enzyme ELISA kits (CEA907Ge; Cloud-Clone, Wuhan, China), serotonin ELISA kits (CEA808Ge; Cloud-Clone, Wuhan, China), brain-derived neurotrophic factor (BDNF) ELISA kits (SEA011Mu; Cloud-Clone, Wuhan, China), Corticosterone (Cort) ELISA kits (CSB-E07969m, CUSABIO, Wuhan China), total mercury standard solution (1 mg/mL, GSB04-1729-2004; National Nonferrous Metals and Electronic Materials Analysis and Testing Center, China), nitric acid (GR, Baiyin Liangyou Chemical Reagent Company, Gansu, China), high purity oxygen (purity ≥99.99%, Xining Laoqian Gas Trading Company, China).

Techniques: Suspension, Solubility, X-ray Diffraction, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Standard Deviation